Characterization of Substance P (SP) Aminoterminal SP (1-7) Binding in Brain Regions and Spinal Cord of the Male Rat Studies on the Interaction with Opioid Related Pathways
نویسنده
چکیده
منابع مشابه
Specific binding of substance P aminoterminal heptapeptide [SP(1-7)] to mouse brain and spinal cord membranes.
Aminoterminal fragments of substance P (SP) have been previously shown to produce effects distinct, and often opposite, from those produced by the C-terminal of SP. The present investigation was initiated to determine whether N-terminal fragments interact at binding sites distinct from the neurokinin-1 (NK-1) receptor where the C-terminal sequence of SP binds with high affinity, and distinct fr...
متن کاملClonidine reduces substance P binding in rat spinal cord membrane preparation.
The effects of clonidine on substance P (SP) binding was investigated using rat brain and spinal cord membrane preparations preincubated with various concentrations of clonidine. [3H]SP specific binding in the spinal cord was significantly decreased with 10(-4) M clonidine, but no effect on binding was seen in the brain. Scatchard analysis of SP binding indicated that Bmax was significantly dep...
متن کاملCommentary Feed - forward inhibition : a novel cellular mechanism for the analgesic effect of substance
Substance P (SP) is a neuropeptide well known for its contribution to pain transmission in the spinal cord, however, less is known about the possible modulatory effects of SP. A new study by Gu and colleagues, published in Molecular Pain (2005, 1:20), describes its potential role in feed-forward inhibition in lamina V of the dorsal horn of the spinal cord. This inhibition seems to function thro...
متن کاملCharacterization and segmental distribution of 125I-Bolton-Hunter-labeled substance P binding sites in rat spinal cord.
Substance P (SP) is widely distributed in the spinal cord and has been implicated as a neurotransmitter in several spinal cord neuronal systems. To investigate SP receptors in the spinal cord, 125I-Bolton-Hunter-SP (125I-BH-SP) was used to identify and characterize spinal cord binding sites for the peptide. The binding of 125I-BH-SP had the following characteristics: high affinity; time, temper...
متن کاملSpinal opioid analgesia: how critical is the regulation of substance P signaling?
Although opioids can reduce stimulus-evoked efflux of Substance P (SP) from nociceptive primary afferents, the consequences of this reduction on spinal cord nociceptive processing has not been studied. Rather than assaying SP release, in the present study we examined the effect of opioids on two postsynaptic measures of SP release, Fos expression and neurokinin-1 (NK-1) receptor internalization...
متن کامل